OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-RAPGEF1 antibodies

Anti-RAPGEF1 TRUEMAB Antibody Clone OTI2F5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA506837 RAPGEF1 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5) 100ul $325 In Stock
LC404802 RAPGEF1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF506837 Carrier-free (BSA/glycerol-free) RAPGEF1 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

spacer
WB(2)
IHC(7)
IF(1)

OriGene Data

ImmunogenFull length human recombinant protein of human RAPGEF1(NP_941372) produced in HEK293T cell.
Clone NameClone OTI2F5 IsotypeIgG1
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:400~4000, IHC 1:150, IF 1:100,
Predicted MW Explanation 122.6 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2
Alternative NameC3G; GRF2
Database LinkNP_941372
Entrez Gene 2889 Human
Entrez Gene 107746 Mouse
Entrez Gene 63881 Rat
Entrez Gene 715272 Monkey
Entrez Gene 491286 Dog
FunctionThis gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008].
Related PathwayDruggable Genome Focal adhesionNeurotrophin signaling pathwayInsulin signaling pathwayRenal cell carcinoma

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAPGEF1 (RC208092, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAPGEF1.
spacer
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RAPGEF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
spacer
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506837)
spacer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506837)
spacer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506837)
spacer
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506837)
spacer
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506837)
spacer
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506837)
spacer
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506837)
spacer
Anti-RAPGEF1 mouse monoclonal antibody (TA506837) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RAPGEF1(RC208092).
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title