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Home Antibody All anti-NEFM antibodies
KO-Validated

Anti-NEFM TRUEMAB Antibody Clone OTI2C4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA506794 NEFM mouse monoclonal antibody, clone OTI2C4 (formerly 2C4) 100ul $325 In Stock
LC417338 NEFM HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF506794 Carrier-free (BSA/glycerol-free) NEFM mouse monoclonal antibody, clone OTI2C4 (formerly 2C4) 100ug $450 3-4 weeks
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WB(2)
IF(2)

OriGene Data

ImmunogenFull length human recombinant protein of human NEFM(NP_005373) produced in HEK293T cell.
Clone NameClone OTI2C4 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB 1:4000, IF 1:100,
Predicted MW Explanation 102.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens neurofilament, medium polypeptide (NEFM), transcript variant 1
Alternative NameNEF3; NF-M; NFM
Database LinkNP_005373
Entrez Gene 4741 Human
Function
Related Pathway Amyotrophic lateral sclerosis (ALS)

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFM (RC224475, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFM.
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WB Image
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and NEFM-Knockout 293T cells (KO, Cat# LC810526) were separated by SDS-PAGE and immunoblotted with anti-NEFM monoclonal antibody TA506794,(1:5000). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
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IF Image
Anti-NEFM mouse monoclonal antibody (TA506794) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEFM(RC224475).
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IF Image
Immunofluorescent staining of HeLa cells using anti-NEFM mouse monoclonal antibody (TA506794).
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