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Anti-BID TRUEMAB Antibody Clone OTI3E6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA506763 BID mouse monoclonal antibody, clone OTI3E6 (formerly 3E6) 100ul $325 In Stock
LC420074 BID HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF506763 Carrier-free (BSA/glycerol-free) BID mouse monoclonal antibody, clone OTI3E6 (formerly 3E6) 100ug $450 8-10 Days
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WB(2)
IF(1)

OriGene Data

ImmunogenFull length human recombinant protein of human BID(NP_001187) produced in HEK293T cell.
Clone NameClone OTI3E6 IsotypeIgG2a
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IF Suggested Dilutions WB 1:400~4000,
Predicted MW Explanation 21.8 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens BH3 interacting domain death agonist (BID), transcript variant 2
Alternative NameFP497
Database LinkNP_001187
Entrez Gene 637 Human
FunctionThis gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008].
Related PathwayDruggable Genome p53 signaling pathwayApoptosisNatural killer cell mediated cytotoxicityAlzheimer's diseaseAmyotrophic lateral sclerosis (ALS)Pathways in cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BID (RC207261, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BID.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BID monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunofluorescent staining of HeLa cells using anti-BID mouse monoclonal antibody (TA506763).
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