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Home Antibody All anti-LGALS3 antibodies
KO-Validated

Anti-LGALS3 TRUEMAB Antibody Clone OTI1A10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA506395 LGALS3 mouse monoclonal antibody, clone OTI1A10 (formerly 1A10) 100ul $325 In Stock
LC400834 LGALS3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF506395 Carrier-free (BSA/glycerol-free) LGALS3 mouse monoclonal antibody, clone OTI1A10 (formerly 1A10) 100ug $450 3-4 weeks
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WB(3)
IF(1)
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Also for LGALS3 (NM_002306)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human LGALS3(NP_002297) produced in HEK293T cell.
Clone NameClone OTI1A10 IsotypeIgG1
Species ReactivityHuman Concentration1.00mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB 1:400 - 1:2000, IF 1:100
Predicted MW Explanation 26 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from cell culture supernatant by affinity chromatography

Reference Data

Target NameHomo sapiens galectin 3 (LGALS3), transcript variant 1
Alternative NameCBP35; GAL3; GALBP; GALIG; L31; LGALS2; MAC2
Database LinkNP_002297
Entrez Gene 3958 Human
FunctionThis gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010].
Related PathwaySecreted Protein

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3 (RC208785, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3.
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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LGALS3 monoclonal antibody at 1:400 dilution (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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WB Image
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and LGALS3-Knockout Hela cells (KO, Cat# LC810128) were separated by SDS-PAGE and immunoblotted with anti-LGALS3 monoclonal antibody TA506395. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.(1:500)
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IF Image
Immunofluorescent staining of HeLa cells using anti-LGALS3 mouse monoclonal antibody (TA506395).
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