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Anti-CD3E TRUEMAB Antibody Clone3E10
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for CD3E (NM_000733)
|Full length human recombinant protein of human CD3E(NP_000724) produced in HEK293T cell.|
|Human , Dog , Monkey
|WB, IHC, IF
|| WB 1:200~4000, IHC 1:150, IF 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens CD3e molecule, epsilon (CD3-TCR complex) (CD3E)|
|IMD18; T3E; TCRE|
Entrez Gene 916 Human
Entrez Gene 699467 Monkey
Entrez Gene 442981 Dog
|The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]. |
Hematopoietic cell lineage
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD3E (RC208276, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD3E.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD3E monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-CD3E monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA506064)
Anti-CD3E mouse monoclonal antibody (TA506064) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD3E(RC208276).