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Home Antibody All anti-CD3E antibodies

Anti-CD3E TRUEMAB Antibody Clone3E10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA506064
  • CD3E (CD3 epsilon) mouse monoclonal antibody, Clone Clone 3E10
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400242) , 20ug Explanation
100ul 379 In Stock
CF506064
  • Carrier-free (BSA/glycerol-free) CD3E mouse monoclonal antibody,clone 3E10
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(3)
IHC(1)
IF(1)
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Also for CD3E (NM_000733)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human CD3E(NP_000724) produced in HEK293T cell.
Clone NameClone3E10 IsotypeIgG2b
Species ReactivityHuman , Dog , Monkey Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:200~4000, IHC 1:150, IF 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens CD3e molecule, epsilon (CD3-TCR complex) (CD3E)
Alternative NameIMD18; T3E; TCRE
Database LinkNP_000724
Entrez Gene 916 Human
Entrez Gene 699467 Monkey
Entrez Gene 442981 Dog
FunctionThe protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008].
Related PathwayTransmembraneDruggable Genome Hematopoietic cell lineageT cell receptor signaling pathwayPrimary immunodeficiency

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD3E (RC208276, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD3E.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD3E monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
WB Image
Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-CD3E monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
IHC Image
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA506064)
IF Image
Anti-CD3E mouse monoclonal antibody (TA506064) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD3E(RC208276).

 

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