Anti-MAP2K1 Antibody, Clone 3H5

Also for MAP2K1 (NM_002755)

OriGene Data

Immunogen	Full length human recombinant protein of human MAP2K1(NP_002746) produced in HEK293T cell.
Clone Name	Clone 3H5	Isotype	IgG2b
Species Reactivity	Human , Dog , Rat , Monkey , Mouse	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	WB 1:200~2000, IHC 1:150, IF 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1)
Alternative Name	MAPKK1; MEK1; MKK1; PRKMK1
Database Link	NP_002746
Function	The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul
Related Pathway	EGFR1 Signaling Pathway Focal Adhesion MAPK signaling pathway Melanogenesis Senescence and Autophagy Toll-like receptor signaling pathway

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K1 (RC218460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K1.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAP2K1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC Image

Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-MAP2K1 mouse monoclonal antibody. (TA505765)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MAP2K1 mouse monoclonal antibody. (TA505765)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAP2K1 mouse monoclonal antibody. (TA505765)

IHC Image

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MAP2K1 mouse monoclonal antibody. (TA505765)

IF Image

Anti-MAP2K1 mouse monoclonal antibody (TA505765) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K1(RC218460).

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-MAP2K1 TrueMAB Antibody Clone 3H5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data