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Home Antibody All anti-MAP2K3 antibodies

Anti-MAP2K3 TRUEMAB Antibody Clone 5D7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA505672
  • MAP2K3 (MEK3) mouse monoclonal antibody, Clone 5D7
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403425) , 20ug Explanation
100ul 325 In Stock
CF505672
  • Carrier-free (BSA/glycerol-free) MAP2K3 mouse monoclonal antibody,clone 5D7
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(3)
IF(1)
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Also for MAP2K3 (NM_145109)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human MAP2K3(NP_659731) produced in HEk293T cell.
Clone NameClone 5D7 IsotypeIgG2a
Species ReactivityHuman , Dog Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:200~4000, IHC 1:150, IF 1:100,
Predicted MW Explanation 39.1 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B
Alternative NameMAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2
Database LinkNP_659731
Entrez Gene 5606 Human
Entrez Gene 489547 Dog
FunctionThe protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008].
Related PathwayProtein KinaseTranscription FactorsDruggable Genome MAPK signaling pathwayToll-like receptor signaling pathwayFc epsilon RI signaling pathwayGnRH signaling pathwayAmyotrophic lateral sclerosis (ALS)

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K3 (RC207115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K3.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAP2K3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-MAP2K3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505672)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAP2K3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505672)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MAP2K3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505672)
IF Image
Anti-MAP2K3 mouse monoclonal antibody (TA505672) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K3(RC207115).

 

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