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Home Antibody All anti-PSMF1 antibodies

Anti-PSMF1 TRUEMAB Antibody Clone 2D7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA505275
  • PSMF1 mouse monoclonal antibody,clone 2D7
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC405875) , 20ug Explanation
100ul 379 In Stock
CF505275
  • Carrier-free (BSA/glycerol-free) PSMF1 mouse monoclonal antibody,clone 2D7
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IF(1)
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Also for PSMF1 (NM_178578)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PSMF1(NP_848693) produced in HEK293T cell.
Clone NameClone 2D7 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IF Suggested Dilutions WB 1:200~2000, IF 1:100,
Predicted MW Explanation 29.6 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens proteasome (prosome, macropain) inhibitor subunit 1 (PI31) (PSMF1), transcript variant 2
Alternative NamePI31
Database LinkNP_848693
Entrez Gene 9491 Human
FunctionThe 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008].
Related Pathway Proteasome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMF1 (RC215482, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMF1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PSMF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IF Image
Anti-PSMF1 mouse monoclonal antibody (TA505275) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMF1(RC215482).

 

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