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Anti-UBE2S TRUEMAB Antibody Clone 4H3
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for UBE2S (NM_014501)
|Full length human recombinant protein of human UBE2S(NP_055316) produced in E.coli.|
|Human , Monkey , Mouse
|WB, IF, FC
|| WB 1:200~2000, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens ubiquitin-conjugating enzyme E2S (UBE2S)|
|E2-EPF; E2EPF; EPF5|
|This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (RC224851, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-UBE2S monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-UBE2S mouse monoclonal antibody (TA505187) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2S(RC224851).
HEK293T cells transfected with either RC224851 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UBE2S antibody(TA505187), and then analyzed by flow cytometry.