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Anti-PHF7 TrueMAB Antibody clone 1B4
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for PHF7 (NM_016483)
|Human recombinant protein fragment corresponding to amino acids 155-381 of human PHF7(NP_057567) produced in E.coli.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
|| WB 1:2000, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens PHD finger protein 7 (PHF7), transcript variant 1|
|HSPC045; HSPC226; NYD-SP6|
|Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. In the testis, this gene is expressed in Sertoli cells but not germ cells. However, this gene is not expressed in a patient who exhibited spermatogenic arrest at the spermatocyte stage. Spermatogenic arrest is an interruption of germ cell differentiation that may result in oligospermia or azoospermia. The proteins encoded by this gene contain plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. Thus this protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Two protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHF7 (RC205192, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHF7.
Anti-PHF7 mouse monoclonal antibody (TA505119) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PHF7(RC205192).
HEK293T cells transfected with either RC205192 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PHF7 antibody(TA505119), and then analyzed by flow cytometry.