Anti-DPH2 Antibody, clone 3A12

Also for DPH2 (NM_001384)

OriGene Data

Immunogen	Full length human recombinant protein of human DPH2(NP_001375) produced in HEK293T cell.
Clone Name	clone 3A12	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens DPH2 homolog (S. cerevisiae) (DPH2), transcript variant 1
Alternative Name	DPH2L2
Database Link	NP_001375
Function	This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPH2 (RC201382, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPH2.

IHC Image

Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-DPH2 mouse monoclonal antibody. (TA504855)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DPH2 mouse monoclonal antibody. (TA504855)

IHC Image

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-DPH2 mouse monoclonal antibody. (TA504855)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DPH2 mouse monoclonal antibody. (TA504855)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-DPH2 mouse monoclonal antibody. (TA504855)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DPH2 mouse monoclonal antibody. (TA504855)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DPH2 mouse monoclonal antibody. (TA504855)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-DPH2 mouse monoclonal antibody. (TA504855)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DPH2 mouse monoclonal antibody. (TA504855)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DPH2 mouse monoclonal antibody. (TA504855)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-DPH2 mouse monoclonal antibody. (TA504855)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DPH2 mouse monoclonal antibody. (TA504855)

IF Image

Anti-DPH2 mouse monoclonal antibody (TA504855) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DPH2(RC201382).

FC Image

HEK293T cells transfected with either RC201382 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DPH2 antibody(TA504855), and then analyzed by flow cytometry.

Anti-DPH2 TrueMAB Antibody clone 3A12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data