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Anti-NONO TrueMAB Antibody clone 3G4
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for NONO (NM_007363)
|Human recombinant protein fragment corresponding to amino acids 184-385 of human NONO(NP_031389) produced in E.coli.|
|Human , Dog , Rat , Monkey , Mouse
||0.5~1.0 mg/ml (Lot Dependent)
|| WB 1:500~2000,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens non-POU domain containing, octamer-binding (NONO), transcript variant 2|
|NMT55; NRB54; P54; P54NRB|
|This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (RC206688, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).