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Anti-DOK7 TRUEMAB Antibody Clone OTI7E2
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for DOK7 (NM_173660)
|Full length human recombinant protein of human DOK7(NP_775931) produced in HEK293T cell.|
|WB, IHC, FC
||WB 1:2000, IHC 1:150, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens docking protein 7 (DOK7), transcript variant 1|
|C4orf25; CMS10; CMS1B|
Entrez Gene 285489 Human
|The protein encoded by this gene is essential for neuromuscular synaptogenesis. The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes. This protein can also induce autophosphorylation of muscle-specific receptor kinase. Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B. Alternative splicing results in multiple transcript variants. [provided by RefSeq].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK7 (RC219267, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK7.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DOK7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DOK7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504816)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DOK7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504816)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DOK7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504816)
HEK293T cells transfected with either RC219267 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DOK7 antibody(TA504816), and then analyzed by flow cytometry.