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Anti-PECAM1 TRUEMAB Antibody Clone OTI2E4
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for PECAM1 (NM_000442)
|Full length human recombinant protein of human PECAM1(NP_000433) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:200 - 1:1000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504812)
Anti-PECAM1 mouse monoclonal antibody (TA504812) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECAM1(RC208654).
HEK293T cells transfected with either RC208654 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PECAM1 antibody(TA504812), and then analyzed by flow cytometry.
Flow cytometric analysis of live Jurkat cells, using anti-PECAM1 antibody (TA504812 at 1:100, red), compare to IgG2b isotype control (Black).
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-PECAM1 monoclonal antibody at 1:200.