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Home Antibody All anti-DOK7 antibodies

Anti-DOK7 TRUEMAB Antibody Clone OTI5G8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA504809 DOK7 mouse monoclonal antibody, clone OTI5G8 (formerly 5G8) 100ul $325 In Stock
LC406543 DOK7 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF504809 Carrier-free (BSA/glycerol-free) DOK7 mouse monoclonal antibody, clone OTI5G8 (formerly 5G8) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type

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WB(2)
IHC(1)
IF(1)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human DOK7(NP_775931) produced in HEK293T cell.
Clone NameClone OTI5G8 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 52.9 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens docking protein 7 (DOK7), transcript variant 1
Alternative NameC4orf25; CMS1B
Database LinkNP_775931
Entrez Gene 285489 Human
FunctionThe protein encoded by this gene is essential for neuromuscular synaptogenesis. The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes. This protein can also induce autophosphorylation of muscle-specific receptor kinase. Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK7 (RC219267, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK7.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-DOK7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DOK7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504809)
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Anti-DOK7 mouse monoclonal antibody (TA504809) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK7(RC219267).
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HEK293T cells transfected with either RC219267 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DOK7 antibody(TA504809), and then analyzed by flow cytometry.
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