HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINE2 (RC204904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINE2.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SERPINE2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC204904 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SERPINE2 antibody(TA504718), and then analyzed by flow cytometry.
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