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Anti-UBE2E3 TRUEMAB Antibody Clone 4B4
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for UBE2E3 (NM_006357)
|Full length human recombinant protein of human UBE2E3(NP_006348) produced in E.coli.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens ubiquitin-conjugating enzyme E2E 3 (UBE2E3), transcript variant 1|
Entrez Gene 10477 Human
|The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]. |
| Ubiquitin mediated proteolysis|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2E3 (RC210328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2E3.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-UBE2E3 monoclonal antibody at 1:200 dilution.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504684)
Anti-UBE2E3 mouse monoclonal antibody (TA504684) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2E3(RC210328).
HEK293T cells transfected with either RC210328 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UBE2E3 antibody(TA504684), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-UBE2E3 antibody(TA504684),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-UBE2E3 antibody(TA504684),(Red), compared to a nonspecific negative control antibody,(Blue).