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Anti-UBE2E3 TRUEMAB Antibody Clone OTI4G4
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for UBE2E3 (NM_006357)
|Full length human recombinant protein of human UBE2E3(NP_006348) produced in E.coli.|
||WB 1:500~2000, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens ubiquitin-conjugating enzyme E2E 3 (UBE2E3), transcript variant 1|
Entrez Gene 10477 Human
Entrez Gene 22193 Mouse
|The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]. |
| Ubiquitin mediated proteolysis|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2E3 (RC210328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2E3.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-UBE2E3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC210328 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UBE2E3 antibody(TA504683), and then analyzed by flow cytometry.