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Anti-PPAT TRUEMAB Antibody Clone 1B2
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for PPAT (NM_002703)
|Human recombinant protein fragment corresponding to amino acids 42-278 of human PPAT(NP_002694) produced in E.coli.|
|Human , Monkey , Mouse
||WB 1:500~2000, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens phosphoribosyl pyrophosphate amidotransferase (PPAT)|
|ATASE; GPAT; PRAT|
|The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq]. COMPLETENESS: complete on the 3' end. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (RC201144, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPAT monoclonal antibody.
HEK293T cells transfected with either RC201144 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPAT antibody(TA504559), and then analyzed by flow cytometry.