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Anti-HLCS TRUEMAB Antibody Clone 1E4
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for HLCS (NM_000411)
|Full length human recombinant protein of human HLCS(NP_000402) produced in HEK293T cell.|
|WB, IHC, FC
||WB 1:2000, IHC 1:150, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant 1|
Entrez Gene 3141 Human
|This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.|
| Biotin metabolismMetabolic pathways|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLCS (RC209197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HLCS.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HLCS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504422)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HLCS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504422)
HEK293T cells transfected with either RC209197 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HLCS antibody(TA504422), and then analyzed by flow cytometry.