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Home Antibody All anti-HLCS antibodies

Anti-HLCS TRUEMAB Antibody Clone OTI1E4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA504422 HLCS mouse monoclonal antibody, clone OTI1E4 (formerly 1E4) 100ul $379 In Stock
LC400145 HLCS HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF504422 Carrier-free (BSA/glycerol-free) HLCS mouse monoclonal antibody, clone OTI1E4 (formerly 1E4) 100ug $450 3-4 weeks
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WB(1)
IHC(2)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human HLCS(NP_000402) produced in HEK293T cell.
Clone NameClone OTI1E4 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 80.6 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant 1
Alternative NameHCS
Database LinkNP_000402
Entrez Gene 3141 Human
FunctionThis gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Related Pathway Biotin metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLCS (RC209197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HLCS.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HLCS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504422)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HLCS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504422)
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HEK293T cells transfected with either RC209197 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HLCS antibody(TA504422), and then analyzed by flow cytometry.
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