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Home Antibody All anti-PPP1R15A antibodies

Anti-PPP1R15A TRUEMAB Antibody clone 1E1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA504359
  • PPP1R15A (GADD34) mouse monoclonal antibody, clone 1E1
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC415358) , 20ug Explanation
100ul 325 In Stock
CF504359
  • Carrier-free (BSA/glycerol-free) PPP1R15A mouse monoclonal antibody,clone 1E1
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IHC(2)
FC(2)
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Also for PPP1R15A (NM_014330)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PPP1R15A(NP_005145) produced in HEK293T cell.
Clone Nameclone 1E1 IsotypeIgG1
Species ReactivityHuman Concentration0.89 mg/ml
Guaranteed Application *WB, IHC, FC Suggested Dilutions WB 1:2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 73.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens protein phosphatase 1, regulatory subunit 15A (PPP1R15A)
Alternative NameGADD34
Database LinkNP_055145
Entrez Gene 23645 Human
FunctionThis gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008].
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R15A (RC200581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R15A.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504359)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504359)
FC Image
HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPP1R15A antibody(TA504359), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-PPP1R15A antibody(TA504359),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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