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Home Antibody All anti-PPP1R15A antibodies

Anti-PPP1R15A TRUEMAB Antibody Clone OTI1E1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA504359 PPP1R15A (GADD34) mouse monoclonal antibody, clone OTI1E1 (formerly 1E1) 100ul $325 In Stock
LC415358 PPP1R15A HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF504359 Carrier-free (BSA/glycerol-free) PPP1R15A mouse monoclonal antibody, clone OTI1E1 (formerly 1E1) 100ug $450 3-4 weeks
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WB(2)
IHC(2)
FC(2)
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Also for PPP1R15A (NM_014330)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PPP1R15A(NP_005145) produced in HEK293T cell.
Clone NameClone OTI1E1 IsotypeIgG1
Species ReactivityHuman Concentration0.89 mg/ml
Guaranteed Application *WB, IHC, FC Suggested Dilutions WB 1:2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 73.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens protein phosphatase 1, regulatory subunit 15A (PPP1R15A)
Alternative NameGADD34
Database LinkNP_055145
Entrez Gene 23645 Human
FunctionThis gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008].
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R15A (RC200581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R15A.
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Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-PPP1R15A monoclonal antibody.(1:200)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504359)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504359)
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HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPP1R15A antibody(TA504359), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Jurkat cells, using anti-PPP1R15A antibody(TA504359),(Red), compared to a nonspecific negative control antibody,(Blue).
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