Anti-PSMB9 Antibody, clone 1D1

Also for PSMB9 (NM_002800)

Gene Name	Homo sapiens proteasome (prosome, macropain) subunit, beta type, 9 (PSMB9)
Synonyms:	beta1i; LMP2; PSMB6i; RING12
Immunogen	Human recombinant protein fragment corresponding to amino acids 21-219 of human PSMB9(NP_002791) produced in E.coli.
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 1D1	Isotype	IgG2a
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Background	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq, Mar
Related Pathway	Cell cycle

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMB9 (RC209001, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMB9.

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PSMB9 mouse monoclonal antibody. (TA504349)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PSMB9 mouse monoclonal antibody. (TA504349)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PSMB9 mouse monoclonal antibody. (TA504349)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMB9 mouse monoclonal antibody. (TA504349)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMB9 mouse monoclonal antibody. (TA504349)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PSMB9 mouse monoclonal antibody. (TA504349)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PSMB9 mouse monoclonal antibody. (TA504349)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PSMB9 mouse monoclonal antibody. (TA504349)

IHC Image

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PSMB9 mouse monoclonal antibody. (TA504349)

IF Image

Anti-PSMB9 mouse monoclonal antibody (TA504349) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMB9(RC209001).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-PSMB9 antibody(TA504349),(Red), compared to a nonspecific negative control antibody,(Blue).

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Anti-PSMB9 TrueMAB Antibody clone 1D1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens