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Anti-CAPN2 TRUEMAB Antibody clone 1F4
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for CAPN2 (NM_001748)
|Full length human recombinant protein of human CAPN2(NP_001739) produced in HEK293T cell.|
|Human , Rat , Monkey , Mouse
|| WB 1:500~2000, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens calpain 2, (m/II) large subunit (CAPN2), transcript variant 1|
|CANP2; CANPL2; CANPml; mCANP|
|The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPN2 (RC205642, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPN2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPN2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC205642 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CAPN2 antibody(TA504345), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-CAPN2 antibody(TA504345),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-CAPN2 antibody(TA504345),(Red), compared to a nonspecific negative control antibody,(Blue).