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Home Antibody All anti-CAPN2 antibodies

Anti-CAPN2 TRUEMAB Antibody clone 1F4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA504345
  • CAPN2 (Calpain 2) mouse monoclonal antibody, clone 1F4
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400662) , 20ug
100ul 379 In Stock
CF504345
  • Carrier-free (BSA/glycerol-free) CAPN2 mouse monoclonal antibody,clone 1F4
100ug $450 3-4 weeks
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WB(2)
FC(3)
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Also for CAPN2 (NM_001748)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human CAPN2(NP_001739) produced in HEK293T cell.
Clone Nameclone 1F4 IsotypeIgG2a
Species ReactivityHuman , Rat , Monkey , Mouse Concentration0.75 mg/ml
Guaranteed Application *WB, FC Suggested Dilutions WB 1:500~2000, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens calpain 2, (m/II) large subunit (CAPN2), transcript variant 1
Alternative NameCANP2; CANPL2; CANPml; mCANP
Database LinkNP_001739
FunctionThe calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009].
Related Pathway
Focal Adhesion

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPN2 (RC205642, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPN2.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPN2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
FC Image
HEK293T cells transfected with either RC205642 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CAPN2 antibody(TA504345), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-CAPN2 antibody(TA504345),(Red), compared to a nonspecific negative control antibody,(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-CAPN2 antibody(TA504345),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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