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Anti-PSMD3 TRUEMAB Antibody clone 2A2
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for PSMD3 (NM_002809)
|Full length human recombinant protein of human PSMD3(NP_002800) produced in HEK293T cell.|
|Human , Dog , Rat , Monkey , Mouse
|| WB 1:500~2000, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 3 (PSMD3)|
|P58; RPN3; S3; TSTA2|
Entrez Gene 5709 Human
Entrez Gene 22123 Mouse
Entrez Gene 287670 Rat
Entrez Gene 698712 Monkey
Entrez Gene 491018 Dog
|The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. [provided by RefSeq, Jul 2008]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD3 (RC202307, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD3.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC202307 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PSMD3 antibody(TA504337), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-PSMD3 antibody(TA504337),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PSMD3 antibody(TA504337),(Red), compared to a nonspecific negative control antibody,(Blue).