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Anti-EPN2 TRUEMAB Antibody Clone OTI1G3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA504296 EPN2 (Epsin 2) mouse monoclonal antibody, clone OTI1G3 (formerly 1G3) 100ul $325 In Stock
CF504296 Carrier-free (BSA/glycerol-free) EPN2 mouse monoclonal antibody, clone OTI1G3 (formerly 1G3) 100ug $450 3-4 weeks
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WB(2)
IHC(6)
IF(1)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human EPN2(NP_055779) produced in HEK293T cell.
Clone NameClone OTI1G3 IsotypeIgG1
Species ReactivityHuman, Monkey Concentration0.73 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 68.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens epsin 2 (EPN2), transcript variant 2
Alternative NameEHB21
Database LinkNP_055779
Entrez Gene 22905 Human
Entrez Gene 702600 Monkey
FunctionThis gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq].
Related Pathway Endocytosis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPN2 (RC213652, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPN2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPN2 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-EPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504296)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-EPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504296)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504296)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504296)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504296)
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-EPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504296)
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Anti-EPN2 mouse monoclonal antibody (TA504296) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EPN2(RC213652).
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Flow cytometric Analysis of Jurkat cells, using anti-EPN2 antibody(TA504296),(Red), compared to a nonspecific negative control antibody,(Blue).
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