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Anti-UCHL1 TRUEMAB Antibody Clone OTI2B5
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for UCHL1 (NM_004181)
|Full length human recombinant protein of human UCHL1(NP_004172) produced in HEK293T cell.|
|Human , Rat , Monkey
|WB, IHC, FC
||WB 1:500~2000, IHC 1:50, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens ubiquitin C-terminal hydrolase L1 (UCHL1)|
|HEL-117; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1|
Entrez Gene 7345 Human
Entrez Gene 29545 Rat
Entrez Gene 701579 Monkey
|The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009].|
|ProteaseDruggable Genome Parkinson's disease|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UCHL1 (RC201803, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UCHL1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UCHL1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504289)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504289)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504289)
HEK293T cells transfected with either RC201803 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UCHL1 antibody(TA504289), and then analyzed by flow cytometry.