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Home Antibody All anti-AKT1 antibodies

Anti-AKT1 TRUEMAB Antibody Clone OTI2B9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA504236
  • AKT1 mouse monoclonal antibody, clone OTI2B9 (formerly 2B9)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401580) , 20ug Explanation
100ul $325 In Stock
CF504236
  • Carrier-free (BSA/glycerol-free) AKT1 mouse monoclonal antibody, clone OTI2B9 (formerly 2B9)
100ug $450 3-4 weeks

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(2)
IF(1)

OriGene Data

ImmunogenFull length human recombinant protein of human AKT1(NP_005154) produced in HEK293T cell.
Clone NameClone OTI2B9 IsotypeIgG2a
Species ReactivityHuman Concentration0.85 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB 1:500~2000, IF 1:100,
Predicted MW Explanation 55.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1
Alternative NameAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Database LinkNP_005154
Entrez Gene 207 Human
FunctionThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (RC220257, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.
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Anti-AKT1 mouse monoclonal antibody (TA504236) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1(RC220257).
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