Anti-AKT1 Antibody, Clone 3B4

Also for AKT1 (NM_005163)

OriGene Data

Immunogen	Full length human recombinant protein of human AKT1(NP_005154) produced in HEK293T cell.
Clone Name	Clone 3B4	Isotype	IgG1
Species Reactivity	Human , Dog , Rat , Monkey , Mouse	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1
Alternative Name	AKT; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Database Link	NP_005154
Function	The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].
Related Pathway	Apoptosis Delta-Notch Signaling Pathway EGFR1 Signaling Pathway Focal Adhesion Hemostasis Jak-STAT signaling pathway MAPK signaling pathway Signaling by GPCR Toll-like receptor signaling pathway Wnt Signaling Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (RC220257, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AKT1 mouse monoclonal antibody. (TA504233)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody. (TA504233)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody. (TA504233)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody. (TA504233)

IF Image

Anti-AKT1 mouse monoclonal antibody (TA504233) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1(RC220257).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-AKT1 antibody(TA504233),(Red), compared to a nonspecific negative control antibody,(Blue).

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Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-AKT1 TrueMAB Antibody Clone 3B4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data