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Anti-AKT1 TrueMAB Antibody Clone 3E3
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for AKT1 (NM_005163)
|Full length humann recombinant protein of human AKT1(NP_005154) produced in HEK293T cell.|
|Human , Dog , Rat , Monkey , Mouse
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1|
|AKT; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA|
|The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].|
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
MAPK signaling pathway
Signaling by GPCR
Toll-like receptor signaling pathway
Wnt Signaling Pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (RC220257, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AKT1 mouse monoclonal antibody. (TA504231)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody. (TA504231)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody. (TA504231)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody. (TA504231)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-AKT1 mouse monoclonal antibody. (TA504231)
Anti-AKT1 mouse monoclonal antibody (TA504231) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1(RC220257).
Flow cytometric Analysis of Jurkat cells, using anti-AKT1 antibody(TA504231),(Red), compared to a nonspecific negative control antibody,(Blue).