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Anti-KCNJ3 TrueMAB Antibody Clone 1G2
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for KCNJ3 (NM_002239)
|Human recombinant protein fragment corresponding to amino acids 279-501 of human KCNJ3(NP_002230) produced in E.coli.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB 1:2000, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), transcript variant 1|
|GIRK1; KGA; KIR3.1|
|Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex. [provided by RefSeq].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KCNJ3 (RC205322, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KCNJ3.
Anti-KCNJ3 mouse monoclonal antibody (TA504198) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KCNJ3(RC205322).
HEK293T cells transfected with either RC205322 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KCNJ3 antibody(TA504198), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-KCNJ3 antibody(TA504198),(Red), compared to a nonspecific negative control antibody,(Blue).