Anti-CST4 Antibody, Clone 2H10

Also for CST4 (NM_001899)

OriGene Data

Immunogen	Full length human recombinant protein of human CST4(NP_001890) produced in HEK293T cell.
Clone Name	Clone 2H10	Isotype	IgG2b
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:2000, IF 1:50~100, FLOW 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens cystatin S (CST4)
Alternative Name	MGC71923
Database Link	NP_001890
Function	The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a type 2 salivary cysteine peptidase inhibitor. The protein is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CST4 (RC209349, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CST4.

IF Image

Anti-CST4 mouse monoclonal antibody (TA504135) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CST4(RC209349).

IF Image

Immunofluorescent staining of HeLa cells using anti-CST4 mouse monoclonal antibody (TA504135).

FC Image

HEK293T cells transfected with either RC209349 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CST4 antibody(TA504135), and then analyzed by flow cytometry.

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OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-CST4 TrueMAB Antibody Clone 2H10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data