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 Home All anti-PFKP antibodies

Anti-PFKP TrueMAB Antibody clone 2F2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Related Products Conjugation/Bulk FAQs
SKU Description Amount Price Availability*  
TA503998
  • Purified PFKP mouse monoclonal antibody,clone 2F2
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400932) , 20ug
 100ul $325 In Stock Add to Shopping Cart
CF503998
  • Carrier-free (BSA/glycerol-free) PFKP mouse monoclonal antibody,clone 2F2
100ug $450 3-4 weeks Add to Shopping Cart
WB(2)
IHC(6)
FC(2)
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Also for PFKP (NM_002627)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens phosphofructokinase, platelet (PFKP), transcript variant 1
Synonyms:PFK-C; PFKF
ImmunogenFull length human recombinant protein of human PFKP(NP_002618) produced in HEK293T cell.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Nameclone 2F2 IsotypeIgG1
Species ReactivityHuman, Monkey Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, FLOW 1:100,
BackgroundThe PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFKP (RC200673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFKP.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PFKP monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PFKP mouse monoclonal antibody. (TA503998)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PFKP mouse monoclonal antibody. (TA503998)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PFKP mouse monoclonal antibody. (TA503998)
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-PFKP mouse monoclonal antibody. (TA503998)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PFKP mouse monoclonal antibody. (TA503998)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PFKP mouse monoclonal antibody. (TA503998)
FC Image
Flow cytometric Analysis of Hela cells, using anti-PFKP antibody(TA503998),(Red), compared to a nonspecific negative control antibody,(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-PFKP antibody(TA503998),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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