Anti-PFKP Antibody, clone 3G8

Also for PFKP (NM_002627)

Gene Name	Homo sapiens phosphofructokinase, platelet (PFKP), transcript variant 1
Synonyms:	PFK-C; PFKF
Immunogen	Full length human recombinant protein of human PFKP(NP_002618) produced in HEK293T cell.
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 3G8	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Background	The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFKP (RC200673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFKP.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PFKP monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PFKP mouse monoclonal antibody. (TA503983)

IHC Image

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-PFKP mouse monoclonal antibody. (TA503983)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PFKP mouse monoclonal antibody. (TA503983)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PFKP mouse monoclonal antibody. (TA503983)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PFKP mouse monoclonal antibody. (TA503983)

IF Image

Anti-PFKP mouse monoclonal antibody (TA503983) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PFKP(RC200673).

FC Image

Flow cytometric Analysis of Hela cells, using anti-PFKP antibody(TA503983),(Red), compared to a nonspecific negative control antibody,(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-PFKP antibody(TA503983),(Red), compared to a nonspecific negative control antibody,(Blue).

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Anti-PFKP TrueMAB Antibody clone 3G8

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