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Home Antibody All anti-NUCB1 antibodies

Anti-NUCB1 TRUEMAB Antibody Clone OTI1A5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503868 NUCB1 (Nucleobindin 1) mouse monoclonal antibody, clone OTI1A5 (formerly 1A5) 100ul
30ul
$325 In Stock
LC416822 NUCB1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503868 Carrier-free (BSA/glycerol-free) NUCB1 mouse monoclonal antibody, clone OTI1A5 (formerly 1A5) 100ug $450 3-4 weeks
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WB(2)
IHC(2)
IF(1)
FC(1)
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Also for NUCB1 (NM_006184)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human NUCB1(NP_006175) produced in HEk293T cell.
Clone NameClone OTI1A5 IsotypeIgG1
Species ReactivityHuman Concentration0.93 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 53.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens nucleobindin 1 (NUCB1)
Alternative NameCALNUC; NUC
Database LinkNP_006175
Entrez Gene 4924 Human
FunctionThis gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq, Jun 2010].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUCB1 (RC201786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1.
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Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-NUCB1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NUCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503868)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NUCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503868)
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Anti-NUCB1 mouse monoclonal antibody (TA503868) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUCB1(RC201786).
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HEK293T cells transfected with either RC201786 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NUCB1 antibody(TA503868), and then analyzed by flow cytometry.
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