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Anti-NUCB1 TRUEMAB Antibody clone 1A5
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for NUCB1 (NM_006184)
|Full length human recombinant protein of human NUCB1(NP_006175) produced in HEk293T cell.|
|WB, IHC, IF, FC
|| WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens nucleobindin 1 (NUCB1)|
Entrez Gene 4924 Human
|This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq, Jun 2010]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUCB1 (RC201786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1.
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-NUCB1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-NUCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503868)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NUCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503868)
Anti-NUCB1 mouse monoclonal antibody (TA503868) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUCB1(RC201786).
HEK293T cells transfected with either RC201786 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NUCB1 antibody(TA503868), and then analyzed by flow cytometry.