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Anti-CAPN9 TRUEMAB Antibody Clone OTI2G1
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for CAPN9 (NM_006615)
|Full length human recombinant protein of human CAPN9(NP_006606) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens calpain 9 (CAPN9), transcript variant 1|
Entrez Gene 10753 Human
|Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].|
|ProteaseDruggable Genome |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPN9 (RC215171, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPN9.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPN9 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CAPN9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503774)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CAPN9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503774)
Anti-CAPN9 mouse monoclonal antibody (TA503774) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CAPN9(RC215171).
Flow cytometric Analysis of Jurkat cells, using anti-CAPN9 antibody(TA503774),(Red), compared to a nonspecific negative control antibody,(Blue).