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Anti-SEC14L2 TRUEMAB Antibody clone 4H2
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for SEC14L2 (NM_012429)
|Full length human recombinant protein of human SEC14L2(NP_036561) produced in HEK293T cell.|
|Human , Dog , Monkey , Mouse
|WB, IHC, IF, FC
||WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1|
|C22orf6; SPF; TAP; TAP1|
Entrez Gene 23541 Human
Entrez Gene 67815 Mouse
Entrez Gene 477539 Dog
|This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq].|
|Transcription Factors |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (RC215994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SEC14L2 monoclonal antibody.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SEC14L2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SEC14L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503723)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-SEC14L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503723)
Anti-SEC14L2 mouse monoclonal antibody (TA503723) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SEC14L2(RC215994).
HEK293T cells transfected with either RC215994 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SEC14L2 antibody(TA503723), and then analyzed by flow cytometry.