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Anti-PPARA TRUEMAB Antibody Clone OTI2G6
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for PPARA (NM_005036)
|Human recombinant protein fragment corresponding to amino acids 145-420 of human PPARA(NP_005027) produced in E.coli.|
|WB, IHC, IF
||WB 1:500, IHC 1:150, IF 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens peroxisome proliferator activated receptor alpha (PPARA), transcript variant 5|
|hPPAR; NR1C1; PPAR; PPARalpha|
Entrez Gene 5465 Human
|Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq].|
|Nuclear Hormone ReceptorTranscription FactorsDruggable Genome PPAR signaling pathwayAdipocytokine signaling pathway|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARA (RC216176, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARA.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPARA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PPARA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503545)
Anti-PPARA mouse monoclonal antibody (TA503545) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA(RC216176).