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Home Antibody All anti-PPARA antibodies

Anti-PPARA TRUEMAB Antibody Clone OTI2E7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503543 PPARA (PPAR alpha) mouse monoclonal antibody, clone OTI2E7 (formerly 2E7) 100ul $325 In Stock
LC401560 PPARA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503543 Carrier-free (BSA/glycerol-free) PPARA mouse monoclonal antibody, clone OTI2E7 (formerly 2E7) 100ug $450 3-4 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IF(1)
FC(1)

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 145-420 of human PPARA(NP_005027) produced in E.coli.
Clone NameClone OTI2E7 IsotypeIgG1
Species ReactivityHuman Concentration0.68 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500, IF 1:100, FLOW 1:100,
Predicted MW Explanation 52 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens peroxisome proliferator-activated receptor alpha (PPARA), transcript variant 5
Alternative NamehPPAR; NR1C1; PPAR; PPARalpha
Database LinkNP_005027
Entrez Gene 5465 Human
FunctionPeroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq].
Related PathwayNuclear Hormone ReceptorTranscription FactorsDruggable Genome PPAR signaling pathwayAdipocytokine signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARA (RC216176, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARA.
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Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-PPARA monoclonal antibody. (1:200)
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Anti-PPARA mouse monoclonal antibody (TA503543) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA(RC216176).
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HEK293T cells transfected with either RC216176 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPARA antibody(TA503543), and then analyzed by flow cytometry.
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