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Home Antibody All anti-ANAPC2 antibodies

Anti-ANAPC2 TRUEMAB Antibody Clone OTI1A6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA503411 ANAPC2 (Apc2) mouse monoclonal antibody, clone OTI1A6 (formerly 1A6) 100ul $379 In Stock
LC402247 ANAPC2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503411 Carrier-free (BSA/glycerol-free) ANAPC2 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6) 100ug $450 3-4 weeks
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WB(3)
IHC(4)
IF(1)
FC(1)
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Also for ANAPC2 (NM_013366)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ANAPC2(NP_037498) produced in HEk293 cell.
Clone NameClone OTI1A6 IsotypeIgG2a
Species ReactivityHuman , Rat , Monkey , Mouse Concentration0.74 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 93.6 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens anaphase promoting complex subunit 2 (ANAPC2)
Alternative NameAPC2
Database LinkNP_037498
Entrez Gene 29882 Human
Entrez Gene 99152 Mouse
Entrez Gene 296558 Rat
Entrez Gene 706384 Monkey
FunctionA large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq].
Related PathwayDruggable Genome Cell cycleOocyte meiosisUbiquitin mediated proteolysisProgesterone-mediated oocyte maturation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANAPC2 (RC207539, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANAPC2 monoclonal antibody.
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Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-ANAPC2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ANAPC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503411)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ANAPC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503411)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ANAPC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503411)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ANAPC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503411)
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Anti-ANAPC2 mouse monoclonal antibody (TA503411) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANAPC2(RC207539).
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HEK293T cells transfected with either RC207539 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ANAPC2 antibody(TA503411), and then analyzed by flow cytometry.
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