Home Antibody All anti-ANAPC2 antibodies
Anti-ANAPC2 TRUEMAB Antibody clone 1H8
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for ANAPC2 (NM_013366)
|Full length human recombinant protein of human ANAPC2(NP_037498) produced in HEk293 cell.|
|Human , Rat , Mouse
|WB, IF, FC
||WB 1:500~2000, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens anaphase promoting complex subunit 2 (ANAPC2)|
|A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq].|
TGF Beta Signaling Pathway
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANAPC2 (RC207539, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANAPC2 monoclonal antibody.
Anti-ANAPC2 mouse monoclonal antibody (TA503407) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANAPC2(RC207539).
HEK293T cells transfected with either RC207539 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ANAPC2 antibody(TA503407), and then analyzed by flow cytometry.