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Home Antibody All anti-COASY antibodies

Anti-COASY TRUEMAB Antibody Clone 3B3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA503344
  • COASY mouse monoclonal antibody,clone 3B3
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403068) , 20ug Explanation
100ul 379 In Stock
CF503344
  • Carrier-free (BSA/glycerol-free) COASY mouse monoclonal antibody,clone 3B3
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IHC(7)
IF(1)
FC(1)
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Also for COASY (NM_025233)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human COASY(NP_079509) produced in HEK293T cell.
Clone NameClone 3B3 IsotypeIgG2a
Species ReactivityHuman Concentration0.75 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 62.1 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens CoA synthase (COASY), transcript variant 1
Alternative NameDPCK; NBIA6; NBP; pOV-2; PPAT; UKR1
Database LinkNP_079509
Entrez Gene 80347 Human
FunctionBiosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM].
Related Pathway Pantothenate and CoA biosynthesisMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COASY (RC220733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COASY.
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503344)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503344)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503344)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503344)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503344)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503344)
IHC Image
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503344)
IF Image
Anti-COASY mouse monoclonal antibody (TA503344) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY COASY(RC220733).
FC Image
HEK293T cells transfected with either RC220733 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-COASY antibody(TA503344), and then analyzed by flow cytometry.

 

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