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Anti-COASY TRUEMAB Antibody Clone OTI2H1
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for COASY (NM_025233)
|Full length human recombinant protein of human COASY(NP_079509) produced in HEK293T cell.|
|WB, IHC, FC
||WB 1:2000, IHC 1:150, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens CoA synthase (COASY), transcript variant 1|
|DPCK; NBIA6; NBP; pOV-2; PPAT; UKR1|
Entrez Gene 80347 Human
|Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 188.8.131.52) and dephospho-CoA kinase (DPCK; EC 184.108.40.206), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM].|
| Pantothenate and CoA biosynthesisMetabolic pathways|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COASY (RC220733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COASY.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503343)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503343)
HEK293T cells transfected with either RC220733 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-COASY antibody(TA503343), and then analyzed by flow cytometry.