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Home Antibody All anti-COASY antibodies

Anti-COASY TRUEMAB Antibody Clone OTI2H1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503343 COASY mouse monoclonal antibody, clone OTI2H1 (formerly 2H1) 100ul $325 In Stock
LC403068 COASY HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503343 Carrier-free (BSA/glycerol-free) COASY mouse monoclonal antibody, clone OTI2H1 (formerly 2H1) 100ug $450 3-4 weeks
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WB(1)
IHC(2)
FC(1)
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Also for COASY (NM_025233)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human COASY(NP_079509) produced in HEK293T cell.
Clone NameClone OTI2H1 IsotypeIgG1
Species ReactivityHuman Concentration0.59 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 62.1 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens CoA synthase (COASY), transcript variant 1
Alternative NameDPCK; NBIA6; NBP; pOV-2; PPAT; UKR1
Database LinkNP_079509
Entrez Gene 80347 Human
FunctionBiosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM].
Related Pathway Pantothenate and CoA biosynthesisMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COASY (RC220733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COASY.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503343)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503343)
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HEK293T cells transfected with either RC220733 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-COASY antibody(TA503343), and then analyzed by flow cytometry.
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