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Home Antibody All anti-LGR5 antibodies

Anti-LGR5 TRUEMAB Antibody clone 2A2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (6) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503316
  • LGR5 mouse monoclonal antibody, clone 2A2
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401213) , 20ug Explanation
100ul 379 In Stock
CF503316
  • Carrier-free (BSA/glycerol-free) LGR5 mouse monoclonal antibody, clone 2A2
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IP(1)
IHC(9)
IF(3)
FC(3)
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Also for LGR5 (NM_003667)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 250-550 of human LGR5 (NP_003658) produced in HEK293T Cell.
Clone Nameclone 2A2 IsotypeIgG1
Species ReactivityHuman , Mouse Concentration0.73 mg/ml
Guaranteed Application *WB, IHC, IF, IP, FC Suggested Dilutions WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100, IP 2ug/500ul
Predicted MW Explanation 99.8 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 1
Alternative NameFEX; GPR49; GPR67; GRP49; HG38
Database LinkNP_003658
Entrez Gene 8549 Human
Entrez Gene 14160 Mouse
Function
Related PathwayGPCRTransmembraneDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (RC212825, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.
WB Image
Figure from citation: Western blot analysis of ß-catenin and non-phospho-ß-catenin in the cytoplasm and the nucleus of si-Ctrl and si-LGR5 treated cell lines. GAPDH was used as an internal control for the cytosolic proteins, and Lamin A/C was used as an internal control for the nuclear proteins. Dilution: 1:500 View Citation
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316)
IHC Image
Figure from citation: Immunohistochemical staining of Lgr5 in normal gastric tissues. The normal gastric tissue, 40×magnified (A) and 400× magnified (B). Yellowspots represent the Lgr5 positive cells that are localized only at base of the glands in normal gastric mucosa. The arrows in the pictures show the immunoreactive cells stained in yellow color. View Citation
IHC Image
Figure from citation: Immunohistochemical analysis of Lgr5 expression in human normal colon biopsies; Lgr5+ cells are localized at the crypt base, where bona fide stem-like cells home; magnification is indicated in the boxes. Dilution: 1:200 View Citation
IHC Image
Figure from citation: Positive correlation between LSD1, c-Myc, ß-catenin and LGR5 expression in human colorectal tumor tissues. Expression levels of LSD1, LGR5, ß-catenin and c-Myc in consecutive sections from normal colon, adenoma and CRC tumor tissues. Dilution: 1:100 View Citation
IF Image
Anti-LGR5 mouse monoclonal antibody (TA503316) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5(RC212825).
IF Image
Immunofluorescent staining of HT29 cells using anti-LGR5 mouse monoclonal antibody (TA503316, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).
IF Image
Figure from citation: Immunocytochemistry of epithelium stem cell phenotype. Isolated dental epithelium following 5-day culture, overwhelmingly expressed Lgr5. Dilution: 1:100 View Citation
IP Image
Immunoprecipitation(IP) of LGR5 by using TrueMab monoclonal anti-LGR5 antibodies (Negative control: IP without adding anti-LGR5 antibody.). For each experiment, 500ul of DDK tagged LGR5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LGR5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
FC Image
HEK293T cells transfected with either RC212825 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGR5 antibody(TA503316), and then analyzed by flow cytometry.
FC Image
Figure from citation: Cytofluorimetric analysis of Lgr5 expression by PKH high cells over 4 weeks of culture. Data are expressed as percent mean values (6 SD) of 5 consecutive experiments. Dilution: 1:400 View Citation
FC Image
Figure from citation: Flow cytometry of the SB cells. The G4 region was further divided into P1 and P2. Lgr5, a stem cell marker, was expressed by 32% of the P2 population. Black: staining with Lgr5; red: staining with the isotype control. View Citation

 

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