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Anti-ATG3 TRUEMAB Antibody Clone OTI3C6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA503301 ATG3 mouse monoclonal antibody, clone OTI3C6 (formerly 3C6) 100ul 325
In Stock
LC411559 ATG3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503301 Carrier-free (BSA/glycerol-free) ATG3 mouse monoclonal antibody, clone OTI3C6 (formerly 3C6) 100ug $450 3-4 weeks
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OriGene Data

ImmunogenFull length human recombinant protein of human ATG3(NP_071933) produced in HEK293T cell.
Clone NameClone OTI3C6 IsotypeIgG2a
Species ReactivityHuman , Dog , Rat , Monkey Concentration0.8 mg/ml
Guaranteed Application *WB, IF, FC Suggested Dilutions WB 1:500~2000, IF 1:100, FLOW 1:100,
Predicted MW Explanation 35.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens autophagy related 3 (ATG3), transcript variant 1
Alternative NameAPG3; APG3-LIKE; APG3L; PC3-96
Database LinkNP_071933
Entrez Gene 64422 Human
Entrez Gene 171415 Rat
Entrez Gene 708305 Monkey
Entrez Gene 478564 Dog
FunctionAutophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM, Mar 2008].
Related Pathway Regulation of autophagy

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG3 (RC203453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG3.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATG3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IF Image
Anti-ATG3 mouse monoclonal antibody (TA503301) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3(RC203453).

FC Image
HEK293T cells transfected with either RC203453 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ATG3 antibody(TA503301), and then analyzed by flow cytometry.


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