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Anti-SIRT5 TRUEMAB Antibody Clone OTI 2E12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503292 SIRT5 mouse monoclonal antibody, clone OTI 2E12 (formerly 2E12) 100ul $325 In Stock
LC415885 SIRT5 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503292 Carrier-free (BSA/glycerol-free) SIRT5 mouse monoclonal antibody, clone OTI 2E12 (formerly 2E12) 100ug $450 3-4 weeks
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WB(1)
IF(1)
FC(1)
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Also for SIRT5 (NM_012241)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human SIRT5 (NP_036373) produced in HEK293T cell.
Clone NameClone OTI 2E12 IsotypeIgG1
Species ReactivityHuman Concentration0.57 mg/ml
Guaranteed Application *WB, IF, FC Suggested Dilutions WB 1:500, FLOW 1:100, IF: 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens sirtuin 5 (SIRT5), transcript variant 1
Alternative NameSIR2L5
Database LinkNP_036373
Entrez Gene 23408 Human
FunctionThis gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2010].
Related PathwayTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.
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Anti-SIRT5 mouse monoclonal antibody (TA503292) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189).
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HEK293T cells transfected with either RC200189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SIRT5 antibody(TA503292), and then analyzed by flow cytometry.
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