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Anti-SMS TRUEMAB Antibody clone 1A7
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Full length human recombinant protein of human SMS (NP_004586) produced in HEK293T cell.|
|WB, IF, FC
|| WB 1:2000, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens spermine synthase (SMS), transcript variant 1|
|MRSR; SPMSY; SpS; SRS|
|This gene encodes a protein belonging to the spermidine/spermin synthase family. Pseudogenes of this gene are located on chromosomes 1, 5, 6 and X. Mutations in this gene are associated with X-linked Snyder-Robinson mental retardation syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMS (RC200619, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMS.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SMS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-SMS mouse monoclonal antibody (TA503095) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMS(RC200619).
HEK293T cells transfected with either RC200619 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SMS antibody(TA503095), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-SMS antibody(TA503095),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-SMS antibody(TA503095),(Red), compared to a nonspecific negative control antibody,(Blue).