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Anti-AP2M1 TRUEMAB Antibody Clone OTI 2F11
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for AP2M1 (NM_001025205)
|Human recombinant protein fragment corresponding to amino acids 97-383 of human AP2M1 (NP_001020376) produced in E.coli.|
|Clone OTI 2F11
|Human , Dog , Rat
|WB, IHC, FC
||WB 1:500~2000, IHC 1:150, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens adaptor related protein complex 2 mu 1 subunit (AP2M1), transcript variant 2|
|AP50; CLAPM1; mu2|
Entrez Gene 1173 Human
Entrez Gene 116563 Rat
Entrez Gene 607139 Dog
|This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].|
|Druggable Genome EndocytosisHuntington's disease|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AP2M1 (RC201377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AP2M1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AP2M1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-AP2M1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503021)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AP2M1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503021)
Flow cytometric Analysis of Jurkat cells, using anti-AP2M1 antibody(TA503021),(Red), compared to a nonspecific negative control antibody,(Blue).