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Anti-AP2M1 TrueMAB Antibody clone 2G2
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for AP2M1 (NM_001025205)
|Human recombinant protein fragment corresponding to amino acids 97-383 of human AP2M1 (NP_001020376) produced in E.coli.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB 1:500~2000, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 2|
|AP50; CLAPM1; mu2|
|This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AP2M1 (RC201377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AP2M1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AP2M1 monoclonal antibody.
HEK293T cells transfected with either RC201377 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AP2M1 antibody(TA503017), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-AP2M1 antibody(TA503017),(Red), compared to a nonspecific negative control antibody,(Blue).