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Home Antibody All anti-AP2M1 antibodies

Anti-AP2M1 TRUEMAB Antibody Clone OTI1F10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503015 AP2M1 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10) 100ul $325 In Stock
LC422484 AP2M1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503015 Carrier-free (BSA/glycerol-free) AP2M1 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10) 100ug $450 3-4 weeks
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WB(2)
IF(1)
FC(1)
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Also for AP2M1 (NM_001025205)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 97-383 of human AP2M1 (NP_001020376) produced in E.coli.
Clone NameClone OTI1F10 IsotypeIgG2a
Species ReactivityHuman Concentration0.87 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:2000, IF 1:100, FLOW 1:100,
Predicted MW Explanation 49.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 2
Alternative NameAP50; CLAPM1; mu2
Database LinkNP_001020376
Entrez Gene 1173 Human
FunctionThis gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related PathwayDruggable Genome EndocytosisHuntington's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AP2M1 (RC201377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AP2M1.
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Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-AP2M1 monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
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Anti-AP2M1 mouse monoclonal antibody (TA503015) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AP2M1(RC201377).
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HEK293T cells transfected with either RC201377 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AP2M1 antibody(TA503015), and then analyzed by flow cytometry.
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