Anti-AK4 Antibody, clone 3B1

Also for AK4 (NM_013410)

OriGene Data

Immunogen	Full length human recombinant protein of human AK4(NP_037542) produced in HEK293 cell.
Clone Name	clone 3B1	Isotype	IgG1
Species Reactivity	Human , Dog , Rat , Monkey , Mouse	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens adenylate kinase 4 (AK4), nuclear gene encoding mitochondrial protein, transcript variant 2
Alternative Name	AK3; AK3L1; AK3L2
Database Link	NP_037542
Function	This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK4 (RC220572, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK4.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK4 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AK4 mouse monoclonal antibody. (TA503011)

IHC Image

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-AK4 mouse monoclonal antibody. (TA503011)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AK4 mouse monoclonal antibody. (TA503011)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-AK4 mouse monoclonal antibody. (TA503011)

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-AK4 mouse monoclonal antibody. (TA503011)

IHC Image

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-AK4 mouse monoclonal antibody. (TA503011)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AK4 mouse monoclonal antibody. (TA503011)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-AK4 mouse monoclonal antibody. (TA503011)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AK4 mouse monoclonal antibody. (TA503011)

IF Image

Anti-AK4 mouse monoclonal antibody (TA503011) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AK4(RC220572).

FC Image

HEK293T cells transfected with either RC220572 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK4 antibody(TA503011), and then analyzed by flow cytometry.

Anti-AK4 TrueMAB Antibody clone 3B1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data